Complete development and multiplication of Cryptosporidium hominis in cell-free culture.

Complete development and multiplication of Cryptosporidium hominis in cell - free 3 culture . 4 5 6

Cultivation of Cryptosporidium in host free cells culture using Iraqi isolate

Cryptosporidiosis is a clinical disease, usually presenting as a gastro-enteritis-like syndrome, caused by infection with protozoan parasites of the Apicomplexan genus Cryptosporidium. Disease ranges in seriousness from mild to severe and signs and symptoms depend on the site of infection and nutritional and immune status of the host Cryptosporidium in vitro was first achieved in endodermal cel...

Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water.

This study compared the three most commonly used assays for detecting Cryptosporidium sp. infections in cell culture: immunofluorescent antibody and microscopy assay (IFA), PCR targeting Cryptosporidium sp.-specific DNA, and reverse transcriptase PCR (RT-PCR) targeting Cryptosporidium sp.-specific mRNA. Monolayers of HCT-8 cells, grown in 8-well chamber slides or 96-well plates, were inoculated...

Complete development of Cryptosporidium parvum in host cell-free culture.

The present study describes the complete in vitro development of Cryptosporidium parvum (cattle genotype) in RPMI-1640 maintenance medium devoid of host cells. This represents the first report in which Cryptosporidium is shown to multiply, develop and complete its life cycle without the need for host cells. Furthermore, cultivation of Cryptosporidium in diphasic medium consisting of a coagulate...

Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry

Until recently, Cryptosporidium was thought to be a single species genus. Molecular studies now showthat there are at least 10 valid species of this parasite. Among them, two morphologically identical species, C.hominis and C. parvum are the most pathogenic identified to date and share 97% of identical genomes.Post-genomic analyses is therefore necessary to explore further the...